Sequence data

All sequence data generated within this study can be obtained via the BioProject accession number PRJNA809118. The dataset contains 30 samples from eggs, larvae, pupae, and adults of the black soldier fly sequenced with bacteria and fungi-specific primers.


Metadata

This table provides all metadata used in this study.

SampleID
Replicate
Group
Label
Condition
Stage
Tissue
Days
Temperature
Target
Primer_set
Forward_primer
Reverse_primer
Forward
Reverse
Reads
Extraction_date
Biomass
EC1_16S
1
EC
Eggs cage
Non-sterilized
Eggs
Eggs
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
EC1_S19_R1_001.fastq.gz
EC1_S19_R2_001.fastq.gz
26053
30.03.2021
0.153
EC2_16S
2
EC
Eggs cage
Non-sterilized
Eggs
Eggs
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
EC2_S20_R1_001.fastq.gz
EC2_S20_R2_001.fastq.gz
33195
30.03.2021
0.090
EC3_16S
3
EC
Eggs cage
Non-sterilized
Eggs
Eggs
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
EC3_S21_R1_001.fastq.gz
EC3_S21_R2_001.fastq.gz
26954
30.03.2021
0.099
FA1_16S
1
FA
Female abdomen
Non-sterilized
Adult
Haemolymph
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
FA1_S28_R1_001.fastq.gz
FA1_S28_R2_001.fastq.gz
32995
30.03.2021
0.140
FA2_16S
2
FA
Female abdomen
Non-sterilized
Adult
Haemolymph
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
FA2_S29_R1_001.fastq.gz
FA2_S29_R2_001.fastq.gz
45569
30.03.2021
0.158
FA3_16S
3
FA
Female abdomen
Non-sterilized
Adult
Haemolymph
39
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
FA3_S30_R1_001.fastq.gz
FA3_S30_R2_001.fastq.gz
36203
30.03.2021
0.102
GS1_16S
1
GS
Larval gut sterile
Sterilized
Larva
Gut
8
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
GS1_S1_R1_001.fastq.gz
GS1_S1_R2_001.fastq.gz
76554
31.03.2021
0.097
GS2_16S
2
GS
Larval gut sterile
Sterilized
Larva
Gut
8
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
GS2_S2_R1_001.fastq.gz
GS2_S2_R2_001.fastq.gz
53758
31.03.2021
0.087
GS3_16S
3
GS
Larval gut sterile
Sterilized
Larva
Gut
8
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
GS3_S3_R1_001.fastq.gz
GS3_S3_R2_001.fastq.gz
51743
31.03.2021
0.090
LH1_16S
1
LH
Larval haemolymph
Non-sterilized
Larva
Haemolymph
8
27
V3V4
341f/802r
CCTACGGGRSGCAGCAG
TACNVGGGTATCTAATCC
LH1_S4_R1_001.fastq.gz
LH1_S4_R2_001.fastq.gz
31554
31.03.2021
0.159
1–10 of 30 rows
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Explanation of metadata variables

This table provides an explanation of the variables presented in the metadata table.

Variable
Description
Format
SampleID
Unique sample identifier
​
Replicate
Number of replicate
​
Group
Sample group
​
Label
Explanation of SampleID
​
Condition
Information about the sterilization of samples
​
Stage
Developmental stage from which the sample was taken
​
Tissue
Tissue from which the sample was obtained
​
Time
Days after the larvae of the first cycle hatched
[days]
Temperature
Rearing temperature
[°C]
Target
Targeted hypervariable region on the 16S/ITS gene
​
Primer_set
Primer set used to target the respective hypervariable region on the 16S/ITS gene
​
Forward_primer
Sequence of forward primer
​
Reverse_primer
Sequence of reverse primer
​
Forward_reads
File name of forward reads
​
Reverse_reads
File name of reverse reads
​
Reads
Number of generated raw reads
​
Extraction_date
Date on which the DNA was extracted
[yyyy-mm-dd]
Biomass
Biomass of the sample from which the DNA was extracted
[g]